Reagents and instruments for immunology, cell biology and molecular biology.

Mouse CORT (Corticosterone) ELISA Kit

Print

Cat# E-EL-0161-48T

Size : 48T

Brand : Elabscience

Product Summary

Sensitivity	1.93 ng/mL
Detection Range	3.12-200 ng/mL
Sample Volume	50 μL
Total Assay Time	2 h 30 min
Reacitivity	Mouse
Specificity	This kit recognizes Mouse CORT in samples.No significant cross-reactivity or interference between Mouse CORT and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse CORT. During the reaction, Mouse CORT in the sample or standard competes with a fixed amount of Mouse CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse CORT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse CORT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Background

Corticosterone is the adrenal steroid, the major glucocorticoid. Glucocorticoid hormones are also known as corticosteroid hormones and are synthesized mainly in the adrenal cortex; however, more recently the enzymes involved in their synthesis have been found in a variety of cells and tissues, including the heart. The effects of these hormones are mediated via both cytoplasmic mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs), which act as ligand-inducible transcription factor. Corticosterone has profound effect on the structure and function of the hippocampus. Brain corticosterone action through the glucocorticoid receptor may involve memory storage. Emotional stress might cause increases in plasma corticosterone.

Research Area

Signal Transduction , Cancer , Epigenetics And Nuclear Signal , Neuroscience , Cardiovascular , Cell Biology , Stem Cells , Metabolism