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Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA plate was coated with purified human IgG kappa, IgM kappa, IgA kappa, IgG lambda, IgM lambda, and IgA lambda. Immunoglobulins were detected with serially diluted Mouse Anti-Human (lambda chain) secondary antibody, clone JDC-12 (HRP).

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against human lambda light chain.

    Immunogen

    Human lambda light chain.

    Host

    Mouse

    Reactivity

    Human

    Specificity

    Human/rhesus lambda.

    Form

    Liquid

    Conjugation

    HRP

    Purification

    Precipitation method and/or chromatography purification

    Isotype

    IgG1, kappa

    Recommend Usage

    ELISA (1:1000-2000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.4 (50% glycerol).

    Storage Instruction

    Store at 4°C.

  • Applications

    Enzyme-linked Immunoabsorbent Assay

    ELISA plate was coated with purified human IgG kappa, IgM kappa, IgA kappa, IgG lambda, IgM lambda, and IgA lambda. Immunoglobulins were detected with serially diluted Mouse Anti-Human (lambda chain) secondary antibody, clone JDC-12 (HRP).
  • Gene Info — IGL@

    Entrez GeneID

    3535

    Protein Accession#

    P0CG04; P0DOY2; P0DOY3; A0M8Q6

    Gene Name

    IGL@

    Gene Alias

    IGL, MGC88804

    Gene Description

    immunoglobulin lambda locus

    Gene Ontology

    Hyperlink

    Gene Summary

    Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the lambda light chain locus. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments and three C segments are known to be incapable of encoding a protein and are considered pseudogenes. The locus also includes several non-immunoglobulin genes, many of which are pseudogenes or are predicted by automated computational analysis or homology to other species. [provided by RefSeq

    Other Designations

    immunoglobulin lambda gene cluster

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