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Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of Mouse Anti-human IgE secondary antibody, clone 4D6 (Cat# MAB12444) under 2 ug/mL working concentration.

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against human IgE.

    Immunogen

    Human IgE whole molecule.

    Host

    Mouse

    Reactivity

    Human

    Specificity

    This product is an IgG2a kappa antibody that was purified from tissue culture. It reacts with human IgE by indirect ELISA and does not react with IgM, IgA or IgG. > 90% pure as determined by page gel.

    Form

    Liquid

    Purification

    Antigen Affinity Purified

    Isotype

    IgG2a

    Recommend Usage

    ELISA (2 ug/mL)
    Immunoprecipitation (2 ug/mg)
    Western Blot (0.05-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.2

    Storage Instruction

    Store at 4°C for 14 days. For long term storage store at -20°C or -80°C.
    Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Mouse Anti-human IgE secondary antibody, clone 4D6 (Cat# MAB12444) under 2 ug/mL working concentration.