Mix-n-Stain™ STORM CF®583R Antibody Labeling Kit
Cat# 92551
Size : 1x50ug
Brand : Biotium
Product Description
Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits allow you to label 50 ug of your antibody with one of Biotium’s STORM CF® Dyes to produce an antibody conjugate with a low 1-2.5 DOL (degree of labeling) optimal for super-resolution STORM (Stochastic Optical Reconstruction Microscopy). Labeling takes just 30 minutes, with minimal hands-on time and no purification.
Features
- Label 50 ug antibody
- Choice of 8 CF® Dye colors ideal for STORM
- CF®583R and CF®597R are novel green-excited STORM dyes
- Labeling optimized to provide low 1-2.5 DOL
- Less than 30 seconds of hands-on time
- 30 minutes total reaction time
- No purification, 100% recovery
Kit components
- Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
- 10X Mix-n-Stain™ Reaction Buffer
- Lyophilized Reactive STORM CF® Dye
- Mix-n-Stain™ Storage Buffer
Superior CF® Dyes for STORM Imaging
These kits feature a choice of 8 CF® Dyes developed with superior brightness and unique photoswitching properties that are ideal for super-resolution imaging by STORM. In addition, these specialized Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits are designed to offer a low degree of labeling (DOL, or dye molecules per antibody molecule) of 1-2.5, which is reported to be optimal for STORM (1). Learn more about CF® Dyes for STORM or our other fluorescent reagents validated for super-resolution microscopy.
Discover More with Green-Excitable CF® Dyes Developed for Multi-Color STORM
High-quality STORM imaging relies heavily on bright fluorescent dyes with excellent photoswitching properties. While there are several commonly used red-excited dyes suitable for STORM imaging such as CF®647, CF®660C and CF®680, generating comparable dyes for other excitation sources to produce quality multi-color STORM images remains a challenge. Biotium scientists have met this challenge by developing novel chemical modifications for green-excited fluorescent dyes to offer optimal performance for STORM super-resolution imaging . This includes CF®583R and CF®597R which were developed specifically for STORM imaging and display exceptional performance for multi-color STORM (2).
See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye. Select the links in the Dye Features column to learn more about each CF® Dye and potential applications.
Other Labeling Kits
We now offer
Mix-n-Stain™ Maxi Antibody Labeling Kits
for labeling 1 mg of antibody with CF® Dyes, andMix-n-Stain™ Nanobody Labeling Kits
. Mix-n-Stain™ kits also are available for labeling antibodies biotin, DNP, or DIG. See our full selection ofMix-n-Stain™ Antibody Labeling Kits.
Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits
| Conjugation | Ex/Em | Size | Catalog No. | Dye Features |
|---|---|---|---|---|
CF®505 | 505/519 nm | 50 ug | 92549 | |
CF®568 | 562/584 nm | 50 ug | 92550 | CF®568 Features |
CF®583R | 585/609 nm | 50 ug | 92551 | CF®583R Features |
CF®597R | 597/619 nm | 50 ug | 92553 | CF®597R Features |
CF®647 | 652/668 nm | 50 ug | 92554 | CF®647 Features |
CF®660C | 667/685 nm | 50 ug | 92555 | CF®660C Features |
CF®680 | 681/698 nm | 50 ug | 92556 | CF®680 Features |
CF®750 | 755/779 nm | 50 ug | 92557 | CF®750 Features |
Antibody & Protein Labeling Kits
| Product Name | Label Options | Labeling Scale | Labeling Time | Features |
|---|---|---|---|---|
Mix-n-Stain™ CF® Dye | CF® Dyes | ≤5-20 ug IgG 20-50 ug IgG 50-100 ug IgG | ~ 15 min. | • Rapid, simple labeling • No purification • Tolerates BSA & other additives • Dyes for super-resolution, spectral flow, & NIR detection |
Mix-n-Stain™ FITC | FITC | |||
Mix-n-Stain™ Cyanine Dye | Cyanine 555 Cyanine 647 | |||
Mix-n-Stain™ Biotin | Biotin | |||
Mix-n-Stain™ Digoxigenin | Digoxygenin (DIG) | |||
Mix-n-Stain™ DNP | DNP | |||
Mix-n-Stain™ Maxi | CF® Dyes Cyanine Dyes | 1 mg IgG | ~ 30 min. | |
Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits | CF® Dyes FITC | 25 ug IgM 100 ug IgM | 15-30 min. | • Rapid, simple labeling • No purification |
Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits | CF® Dyes | 50 ug IgG | ~ 30 min. | • Optimized to provide low 1-2.5 DOL • No purification |
Mix-n-Stain™ | CF® Dyes | 5-20 ug Nanobody® 20-50 ug Nanobody® | ~ 30 min. | • Optimized for Nanobodies® • Tolerates BSA & other additives |
Mix-n-Stain™ | CF® Dyes | 0.1 umol small ligand | ~ 30 min. | • Label SNAP®, CLIP™, HaloTag® & other small ligands • Dye options for surface or intracellular targets |
VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging | Near-IR CF® Dyes | 3 x 1 mg IgG | ~ 2 hours | • Dyes, buffers, & spin vials for labeling + purification • Sterile syringes, filters and storage vials for in vivo use |
CF® Dye & Biotin SE | CF® Dyes Biotin | 3 x 1 mg protein | ~ 2 hours | • Dyes, buffers, & spin vials for labeling + purification |
CF is a registered trademark of Biotium, Inc.
References
- Proc. SPIE 9331, (2015),
DOI: 10.1117/12.2083209
- Angew. Chem. Int. Ed. 9331, e202113612, (2021),
DOI: 10.1002/ANIE.202113612
Download a list of
Mix-n-Stain™ references
.Download a list of
CF® Dye references
.