Methylene blue (trihydrate) [7220-79-3]
Cat# HY-B1359-1ml
Size : 10mM/1mL
Brand : MedChemExpress
Methylene blue trihydrate (C.I. Basic Blue 9 trihydrate) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue trihydrate is a vasopressor and is often used as a dye in several medical procedures. Methylene blue trihydrate has antinociception, antimalarial, antidepressant and anxiolytic activity effects. Methylene Blue trihydrate has the potential for methemoglobinemias, neurodegenerative disorders and ifosfamide-induced encephalopathytreatment.
For research use only. We do not sell to patients.
Methylene blue trihydrate Chemical Structure
CAS No. : 7220-79-3
This product is a controlled substance and not for sale in your territory.
Based on 14 publication(s) in Google Scholar
Other Forms of Methylene blue trihydrate:
- Methylene Blue In-stock
- Methylene blue hydrate Get quote
View All Monoamine Oxidase Isoform Specific Products:
View All NO Synthase Isoform Specific Products:
View All Parasite Isoform Specific Products:
Description |
Methylene blue trihydrate (C.I. Basic Blue 9 trihydrate) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue trihydrate is a vasopressor and is often used as a dye in several medical procedures. Methylene blue trihydrate has antinociception, antimalarial, antidepressant and anxiolytic activity effects. Methylene Blue trihydrate has the potential for methemoglobinemias, neurodegenerative disorders and ifosfamide-induced encephalopathytreatment[1][2][3]. |
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IC50 & Target |
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Cellular Effect |
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In Vitro |
By acting as an alternative electron acceptor/donor Methylene blue restores mitochondrial function, improves neuronal energy production and inhibits the formation of superoxide[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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In Vivo |
Methylene Blue (1, 5, and 25 μg/rat) significantly decreases sevoflurane minimum alveolar anesthetic concentration (MAC) and brain cyclic guanosine monophosphate (cGMP) content in a dose-dependent manner in male Sprague-Dawley rats (7-week-old, 200-250 g)[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Clinical Trial |
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Molecular Weight |
373.90 |
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Formula |
C16H24ClN3O3S |
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CAS No. |
7220-79-3 |
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Appearance |
Solid |
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Color |
Light brown to black |
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SMILES |
CN(C1=CC(SC2=C/C(C=CC2=N3)=[N+](C)\C)=C3C=C1)C.O.[Cl-] |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
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Solvent & Solubility |
In Vitro:
DMSO : 100 mg/mL (267.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: ≥98.0% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 2.6745 mL | 13.3726 mL | 26.7451 mL | 66.8628 mL |
5 mM | 0.5349 mL | 2.6745 mL | 5.3490 mL | 13.3726 mL | |
10 mM | 0.2675 mL | 1.3373 mL | 2.6745 mL | 6.6863 mL | |
15 mM | 0.1783 mL | 0.8915 mL | 1.7830 mL | 4.4575 mL | |
20 mM | 0.1337 mL | 0.6686 mL | 1.3373 mL | 3.3431 mL | |
25 mM | 0.1070 mL | 0.5349 mL | 1.0698 mL | 2.6745 mL | |
30 mM | 0.0892 mL | 0.4458 mL | 0.8915 mL | 2.2288 mL | |
40 mM | 0.0669 mL | 0.3343 mL | 0.6686 mL | 1.6716 mL | |
50 mM | 0.0535 mL | 0.2675 mL | 0.5349 mL | 1.3373 mL | |
60 mM | 0.0446 mL | 0.2229 mL | 0.4458 mL | 1.1144 mL | |
80 mM | 0.0334 mL | 0.1672 mL | 0.3343 mL | 0.8358 mL | |
100 mM | 0.0267 mL | 0.1337 mL | 0.2675 mL | 0.6686 mL |
Methylene blue trihydrate Related Classifications
- GPCR/G Protein Neuronal Signaling Immunology/Inflammation Anti-infection
- Guanylate Cyclase Monoamine Oxidase NO Synthase Parasite