Methylene blue (trihydrate) [7220-79-3]
Cat# HY-B1359-100mg
Size : 100mg
Brand : MedChemExpress
Fish Insulin-like growth factor II(IGF2) ELISA kit
| Code | CSB-EL011088FI |
| Size | 96T,5×96T,10×96T |
| Price | or |
| Trial Size | 24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. |
Product Details
Target Name
insulin-like growth factor 2 (somatomedin A)
Alternative Names
igf2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Erythrotropin ELISA Kit
Abbreviation
Uniprot No.
Species
Fish
Sample Types
serum, plasma
Detection Range
62.5 pg/mL-1000 pg/mL
Sensitivity
31.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 91 | |||||
| Range % | 87-102 | ||||||
| 1:200 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
| 1:400 | Average % | 96 | |||||
| Range % | 88-106 | ||||||
| 1:800 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
Recovery
| The recovery of fish IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 90-102 | |||||
| EDTA plasma (n=4) | 93 | 85-102 | |||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description
This Fish IGF2 ELISA Kit was designed for the quantitative measurement of Fish IGF2 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 62.5 pg/mL-1000 pg/mL and the sensitivity is 31.25 pg/mL.
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for integrin which is required for IGF2 signaling.
Subcellular Location
Secreted.
Protein Families
Insulin family
Database Links
UniGene:
