Images
Flow Cytometry
Application

Flow Cytometry

Flow cytometric analysis of HEK 293T cells with MAP2K4 (phospho S257) monoclonal antibody, clone C5 (PE) (Cat # MAB23473). Unstained and untreated cells as negative control (blue) or treated with lambda phosphatase (red) or treated with UV (100 mJ/cm^2)+TPA (200nM, 1 hr recovery ; green).

  • Specifications

    Product Description

    Rabbit monoclonal antibody raised against synthetic phosphopeptide of human MAP2K4.

    Immunogen

    A synthetic phosphopeptide corresponding to residues surrounding S257 of human MAP2K4.

    Host

    Rabbit

    Reactivity

    Human, Macaque, Mouse

    Form

    Liquid

    Conjugation

    PE

    Purification

    Protein A/G purification

    Isotype

    IgG1, kappa

    Recommend Usage

    Flow Cytometry (5 uL/106 cells)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.4 (0.2% BSA, 0.09% sodium azide).

    Storage Instruction

    Store at 4°C.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Flow Cytometry

    Flow cytometric analysis of HEK 293T cells with MAP2K4 (phospho S257) monoclonal antibody, clone C5 (PE) (Cat # MAB23473). Unstained and untreated cells as negative control (blue) or treated with lambda phosphatase (red) or treated with UV (100 mJ/cm^2)+TPA (200nM, 1 hr recovery ; green).
  • Gene Info — MAP2K4

    Entrez GeneID

    6416

    Gene Name

    MAP2K4

    Gene Alias

    JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SEK1, SERK1

    Gene Description

    mitogen-activated protein kinase kinase 4

    Omim ID

    601335

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver. [provided by RefSeq

    Other Designations

    JNK activating kinase 1|JNK-activated kinase 1|MAP kinase kinase 4|MAPK/ERK kinase 4|SAPK/ERK kinase 1|c-Jun N-terminal kinase kinase 1|dual specificity mitogen-activated protein kinase kinase 4

  • Interactomes
  • Pathways
  • Diseases