Reagents and instruments for immunology, cell biology and molecular biology.

Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

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Cat# E-BC-K025-M-500

Size : 500Assays

Brand : Elabscience

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Detection Principle	MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Synonyms	MDA
Sample Type	Serum,Plasma,Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (530-540 nm, optimum wavelength: 532 nm)
Research Area	Oxidative Stress , Ferroptosis
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1.13 μmol/L
Detection Range	2.92-40 μmol/L
Precision	inter-assay CV: 7.2% \| intra-assay CV: 4.1%
Assay Time	1 h 10 min

Cat.No.	Product Name	Sizes
E-BC-K020-M	Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)	500Assays , 96T , 48T
E-BC-K031-M	Catalase (CAT) Activity Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Human plasma	1
Rat serum	1
Rat plasma	1
Mouse serum	1
Mouse plasma	1
10% Rat heart tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat spleen tissue homogenate	1
10% Rat lung tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat brain tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.