Lactate Dehydrogenase (LDH) Activity Assay Kit (WST-8 method)

Cat# E-BC-K766-M-96T

Size : 96T

Brand : Elabscience

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Detection Principle Lactate dehydrogenase (LDH) is an oxidoreductase. LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. LDH is composed of four subunits (tetramer). The two most common subunits are the LDH-M and LDH-H protein. LDH is released into the blood by cells after tissue damage or erythrocyte hemolysis. Extracellular LDH activity is used to detect cell damage or cell death.
Synonyms LDH
Sample Type Serum,Plasma,Animal tissue,Cell culture supernatant
Detection Method Colorimetric method
Detection Instrument Microplate reader (450 nm)
Research Area Glycolysis And Lipid Metabolism ,  Liver And Renal Function ,  Energy Metabolism
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.11 U/L
Detection Range 0.11-39.9 U/L
Precision inter-assay CV: 2.3% | intra-assay CV: 2.3%
Assay Time 30 min
Cat.No. Product Name Sizes
E-BC-K008-M Monoamine Oxidase (MAO) Activity Assay Kit 96T , 48T
E-BC-K235-M Alanine Aminotransferase (ALT/GPT) Activity Assay Kit 96T , 48T
E-BC-K236-M Aspartate Aminotransferase (AST/GOT) Activity Assay Kit 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 10-20
Dog serum 10-20
Mouse serum 50-100
Cynomolgus monkey serum 10-20
10% Rat spleen tissue homogenate 150-250
10% Rat liver tissue homogenate 250-350
10% Rat kidney tissue homogenate 250-350
10% Rat lung tissue homogenate 250-350

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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