Reagents and instruments for immunology, cell biology and molecular biology.

L-Lactic Acid (LA) Colorimetric Assay Kit (Whole Blood Samples)

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Cat# E-BC-K043-S-50

Size : 50Assays

Brand : Elabscience

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Detection Principle	Using NAD⁺ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD⁺ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm.
Sample Type	Whole blood
Detection Method	Colorimetric method
Detection Instrument	Spectrophotometer (530 nm)
Research Area	Glycolysis And Lipid Metabolism
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.14 mmol/L
Detection Range	0.14-7.0 mmol/L
Precision	inter-assay CV: 0.02 \| intra-assay CV: 1.6%
Sample Volume	0.05 mL
Assay Time	1 h

Cat.No.	Product Name	Sizes
E-BC-K044-S	L-Lactic Acid (LA) Colorimetric Assay Kit	100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Rabbit whole blood	1-2
Mouse whole blood	1-2
Rat whole blood	1-2

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.