Kit SNPXplex®

Cat# 99901

Size : 130reactions


 

SNPXplex® - Identity vigilance kit for NGS

The SNPXplex identity verification kit is a powerful tool for Next generation sequencing, NGS). It enables simultaneous genotyping of 15 single nucleotide polymorphisms (SNPs) using allele-specific fluorescent multiplex PCR, along with sex determination. SNPXplex is suitable for various applications, including targeted panels, exomes, and genomes.

SNPXplex: Identity Verification Kit for High-Throughput Sequencing

Recommendations for good practice in high-throughput sequencing agree on the need for an identity verification technique, based on the comparison of the genotypes of several polymorphisms, with the genotypes being obtained independently by high-throughput sequencing (next generation sequencing, NGS) and by a second technique. The SNPXplex is an identity verification kit enabling the simultaneous genotyping of 15 single nucleotide polymorphisms (SNPs) by allele-specific fluorescent multiplex PCR and sex determination.

In use since 2017, more than 100,000 NGS sequencing results have already been secured. A technology patented by Assistance Publique - Hôpitaux de Paris (APHP) based on the comparison of genotyping results (NGS vs SNPXplex®) for several polymorphisms.
 

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Key features :

Simple, rapid protocol

  • Single, ready-to-use reagent
  • Simultaneous genotyping of 15 single nucleotide polymorphisms (SNPs) by allele-specific fluorescent multiplex PCR + determination of sex ⇒profile
  • Risk of having two patients with the same profile in a series of 96 samples:
    • <5% for all origins combined 
    • <0.2% if of European origin

Can be used with :

  • Targeted panels, exomes and genomes
  • Capture and amplicon

Instruments required :

  • Thermal cycler
  • Capillary sequencer (6-FAM™ detection)

Content 

  • Reagent : SNPXplex 
  • Volume: 1200 µl

Storage: 

  • Reagents can be stored at -20°C. Reagents can be stored at room temperature for up to 8 days, protected from light.

Comparison of SNPXplex vs NGS genotyping results and genoidentity testing

SNPXplex genotyping data can be exported via the GeneMapper™ Export function.

Use local resources to:

  • Compare SNPXplex genotyping results to high-throughput sequencing results on a patient-by-patient basis.
  • Search all patients in the series for possible genoidentity between two patients.


To learn more : 

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Cat#
Description
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Price Bef. VAT
99900
 10reactions