Reagents and instruments for immunology, cell biology and molecular biology.

Keratin 10 (BSA & Azide Free) (FITC)

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Cat# K0199-12X-FITC-100ul

Size : 100ul

Brand : US Biological

K0199-12X-FITC Rabbit Anti-Keratin 10 (BSA & Azide Free) (FITC)

Clone Type

Polyclonal

Host

mouse

Source

human

Isotype

IgG1

Grade

Affinity Purified

Applications

IF IHC WB

Crossreactivity

Ca Fe Hu Rt

Shipping Temp

Blue Ice

Storage Temp

-20°C

Keratin 10 (CK 10) is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK 10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. Expression of CK 10 is related to maturation of malignant keratinocytes, being preferentially detected in more differentiated parts.||Applications: Immunofluorescence, Western Blotting, Immunohistochemistry.||Recommended Dilution:|Western Blotting: 1ug/ml |Immunohistochemistry: 2-4ug/ml. Frozen & formalin/paraffin. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.|Optimal dilutions to be determined by the researcher.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: 0.N.354 (DE-K10)|Host: mouse|Source: human|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein G affinity chromatography.|Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium. Cellular Localization: Cytoplasmic.|Specificity: Recognizes human Keratin 10 at 56.5kD. Species Crossreactivity: rat, canine and feline.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Cytoskeletal preparation extracted from human ectocervical epithelium. Cellular Localization: Cytoplasmic.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

Purity

Purified by Protein G affinity chromatography.

Specificity

Recognizes human Keratin 10 at 56.5kD. Species Crossreactivity: rat, canine and feline.

References

Ivanyi D; Ansink A; Groeneveld E; Hageman PC; Mooi WJ; Heintz AP. New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva. Journal of Pathology, 1989, 159:7-12.