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Western Blot (Cell lysate)
Note

Application Data with Unconjugated Antibody

Western Blot (Cell lysate)

Western blot analysis of 1:1000 diluted human cell lysate (300 ug/gel) with KDEL polyclonal antibody (PAB28964) at 1:500 dilution.

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic peptide of KDEL.

    Immunogen

    A synthetic peptide corresponding to KDEL.

    Host

    Rabbit

    Reactivity

    Human, Mouse, Rat

    Specificity

    Recognizes KDEL proteins, Hsp90b1, Hspa5, PDI and Calr. It may also see PDIA3 and Erp29.

    Form

    Liquid

    Conjugation

    APC

    Purification

    Affinity purification

    Recommend Usage

    Immunohistochemistry
    Western blot (1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.2 (50% glycerol, 0.09% sodium azide)

    Storage Instruction

    Store at 4°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    Application Data with Unconjugated Antibody

    Western Blot (Cell lysate)

    Western blot analysis of 1:1000 diluted human cell lysate (300 ug/gel) with KDEL polyclonal antibody (PAB28964) at 1:500 dilution.

  • Applications

    Western Blot

    Immunohistochemistry