The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
- Specifications
Product Description
Iron Assay Kit is a quantitative colorimetric iron determination at 590 nm.This kit is designed to measure total iron directly in serum without any pretreatment. The improved method utilizes a chromogen that forms a blue colored complex specifically with Fe2+. Fe3+ in the sample is reduced to Fe2+, thus allowing the assay for total iron concentration. The intensity of the color, measured at 590 nm, is directly proportional to the iron concentration in the sample.
Suitable Sample
Biological (e.g. serum) and environmental samples.
Sample Volume
50 uL (using 96-well plate)
250 uL (using cuvette)Detection Method
Colorimetric
Assay Type
Quantitative
Calibration Range
27 ug/dL (4.8 uM) to 1,000 ug/dL (179 uM) in 96-well plate assay.
Regulation Status
For research use only (RUO)
Quality Control Testing
Standard Curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store reagent A at room temperature and other reagents at 4°C.
- Applications
Functional Study
- Publication Reference
- VHL-recruiting PROTAC attenuates renal fibrosis and preserves renal function via simultaneous degradation of Smad3 and stabilization of HIF-2α.
Jiayi Yang, Yuyi Ruan, Dan Wang, Jinjin Fan, Ning Luo, Huiting Chen, Xiaoyan Li, Wei Chen, Xin Wang.
Cell & Bioscience 2022 Dec; 12(1):203.
Application：Func, Mouse , Mouse serum.
- VHL-recruiting PROTAC attenuates renal fibrosis and preserves renal function via simultaneous degradation of Smad3 and stabilization of HIF-2α.