Integrin alpha 6 (ITGA6) rat monoclonal antibody, clone NKI-GoH3, PE
Specifications
Product Data
Clone Name
NKI-GoH3
Application
FC
Recommended Dilution
Flow Cytometry: Neat - 1/5; Use 10µl of the suggested working dilution to label 10e6 platelets in 100µl.
Reactivity
Canine, Human, Monkey, Mouse, Porcine
Antibody Host
Rat
Isotype
IgG2a
Clonality
Monoclonal
Immunogen
BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line.
Specificity
This antibody recognises CD49F (VLA-6 alpha chain). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone GoH3 specifically blocks the binding of cells to the E8 fragment of laminin. We recommend the use of SM038Afor this purpose.
Buffer
PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: PE State: Lyophilized purified IgG Label: R. Phycoerythrin (RPE)
Reconstitution Method
Restore with 1.0 ml distilled water.
Purification
Affinity chromatography on Protein G
Conjugation
PE
Storage
Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE!
CD49F (VLA-6 alpha chain)is a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
