IgM Murine Negative Isotype Control

Cat# I1905-26C-1ml

Size : 1ml

Brand : US Biological

Request more information



I1905-26C IgM Murine Negative Isotype Control

Clone Type
Polyclonal
Isotype
IgM
Grade
Purified
Applications
FC
Shipping Temp
Blue Ice
Storage Temp
-20°C

Isotype Controls:|Isotype controls are a type of negative control designed to measure the level of non-specific background signal caused by primary antibodies, based upon the tissue type of the sample. Non-specific binding of primary antibodies are typically caused by the following reasons:|• Non-specific Fc receptor binding to cells |• Non-specific antibody interactions with cellular proteins or fluorochromes|• Non-specific binding of the antibody or fluorochromes to cellular components |• Cell autofluorescence||Usually, the background signal is the result of immunoglobulins binding non-specifically to Fc receptors present on the cell surface. For example, antibodies raised in mice, particularly those of the IgG2a isotype, will bind strongly to some human leukocytes regardless of the test antibody specificity. In this case you would need a mouse IgG2a isotype control for use with human cells or tissues. Isotype controls are most commonly used in flow cytometry experiments, but may also be used in immunohistochemistry. ||Selecting an Isotype Control:|Typically, an isotype control is matched to the host species and isotype of your specific primary antibody, the heavy chain (IgA, IgG, IgD, IgE, or IgM), subclass and light chain (kappa, lambda) class of the primary antibody;  the same fluorochrome (PE, APC, etc.), and have the same F:P ratio. F:P is a measurement of how many fluorescent molecules are present on each antibody.||When using directly labeled primary antibodies, it is also necessary to make sure that the isotype control is conjugated to the same fluorochrome or label as the test antibody. It is not sufficient to use one with a spectrally similar fluorochrome.||Non-specific Binding:|To avoid non-specific binding, you also need to block Fc receptors on cell types, such as spleen cells, with FcR blocking reagents. Isotype controls should be used at the same concentration/dilution as the primary antibody. Isotype controls are mainly used to determine if the staining is real. Apart from isotype controls, unstained cells should always be included in the experimental set-up to monitor autofluorescence.||Applications for Isotype Controls:|Although isotype controls are mainly used in flow cytometry, they can be used as standard blocking agents and protein coating agents for other applications including immunofluorescence, immunocytochemistry, western blotting, and ELISA.||I1905-26C is an isotype matched negative control to determine the level of non-specific binding of IgM monoclonal antibodies to human leukocytes.||Applications:|Suitable for use in Flow Cytometry. Other applications have not been tested.||Recommended Dilutions:|Flow Cytometry: Neat. Use 10ul to label 1x10e6 cells or 100ul whole blood.|Optimal dilutions to be determined by the researcher.||Recommended Secondary Reagents:|I1905-28A: IgM (X-Adsorbed) (HRP) Pab Gt x Mo|I1905-04A: IgM (X-Adsorbed) (PE) Pab Gt x Mo|I1905-18D: IgM (X-Adsorbed) (FITC) Pab Gt x Mo||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Purity: Purified by ammonium sulfate precipitation from tissue culture supernatant.|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.|Specificity: Does not recognize human antigens.|Recognizes a rat cell surface marker therefore cannot be used as a negative control for rat.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation from tissue culture supernatant.
Specificity
Does not recognize human antigens.|Recognizes a rat cell surface marker therefore cannot be used as a negative control for rat.