IgM, Murine Isotype Control

The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10ug/ml of incubation mixture), change the conditions of your experiment to reduce the background.||Applications:|Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA. Other applications not tested.||Recommended Dilutions:|Immunohistochemistry: frozen and paraffin|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.