Reagents and instruments for immunology, cell biology and molecular biology.

IgA, alpha (FITC)

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Cat# I1890-05K-1mg

Size : 1mg

Brand : US Biological

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I1890-05K IgA, alpha (FITC)

Clone Type

Polyclonal

Host

goat

Source

rat

Isotype

IgG

Grade

Affinity Purified

Applications

FLISA IF WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications: |Suitable for use in Immunfluorescence and FLISA. Other applications have not been tested.||Recommended Dilutions: |Immunofluorescence Microscopy: 1:1000-1:5000|FLISA: 1:10,000-1:50,000|Optimal dilutions to be determined by the researcher.||Absorption Wavelength: 495nm||Emission Wavelength: 528nm||Fluorochrome/Protein Ratio: |9.4 moles FITC per mole of Goat IgG||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Applications

Product Type: Pab|Isotype: IgG|Host: goat|Source: rat|Concentration: ~1mg/ml (after reconstitution)|Form: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC) (MW: 390 daltons). Reconstitute in 1ml sterile ddH2O. |Purity: Purified by immunoaffinity chromatography .|Immunogen: Rat IgA alpha heavy chain|Specificity: Recognizes rat IgA, alpha. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, rat IgA and rat serum. Shows <1% crossreactivity in ELISA against other rat heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Rat IgA alpha heavy chain

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC) (MW: 390 daltons). Reconstitute in 1ml sterile ddH2O.

Purity

Purified by immunoaffinity chromatography .

Specificity

Recognizes rat IgA, alpha. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, rat IgA and rat serum. Shows <1% crossreactivity in ELISA against other rat heavy or light chain isotypes.