Human TNF-a ELISA Kit (96-well)

Cat# EA100044

Size : 1x96wells

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Human TNF-a ELISA Kit (96-well)

SKU
EA100044
Human TNF-a ELISA Kit (96-well)
Specifications
Product Data
ACCN NM_000594
Assay Length 3 hours
Assay Platform 96-well plate
Assay Type Solid Phase Sandwich ELISA
Assay Type Sandwich
Background

The prototype ligand of the TNF superfamily, TNF-./TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis . Human cells known to express TNF-. include B cells, colonic columnar epithelial cells, NK and CD3+CD56+ hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4+ and CD8+ T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.

It is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids (aa) in length . It contains a 30 aa cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region . TNF-. is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNF-. (sTNF-. is released from the C-terminus of the transmembrane protein through the activity of TNF-.-converting enzyme (TACE), a membrane-bound disintegrin metalloproteinase .

TNF-. is reported to promote inflammatory cell infiltration by upregulating leukocyte adhesion molecules on endothelial cells, serve as a chemotactic agent for monocytes, and activate phagocyte killing mechanisms . Deficiencies in either TNF-. or its receptors can increase susceptibility to infection by intracellular pathogens . TNF- may also play a role in lymphoid tissue development. Knockout mice lack splenic B cell follicles and the ability to form germinal centers . Other potential physiological roles for TNF-. and its receptors include regulating the differentiation of hematopoietic stem and progenitor cells .

TNF-. has been implicated in a number of pathophysiological processes. It is associated with unregulated pro-inflammatory activity and is thought to be a critical mediator of endotoxin-induced septic shock . Cachexia (or whole body wasting) has also been associated with long-term circulating TNF-.. Other disorders with potential TNF-. involvement include asthma), type 2 diabetes, CrohnÂ?s disease, and rheumatoid arthritis.

Components Aluminium pouches with a Microwell Plate coated with antibody to human TNF-a(8x12)
2 vials human TNF-a Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human TNF-a antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
4 pieces Adhesive Films
package insert
Curve Range 15.625-1000pg/ml
Format 96-well strip plate
Gene Symbol TNF
Interference No significant interference observed with available related molecules.
Locus ID 7124
Reactivity Human
Reactivity Human
Reactivity Human
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Sensitivity 7pg/mL
Signal Colorimetric
Species Human
Specificity Natural and recombinant Human TNF-a Ligand
Target Symbol TNF
New No
Description Human TNF-a ELISA Kit (96-well)

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Cat#
Description
Cond.
Price Bef. VAT
EA100030
 1x96wells