General Info
Applications: | CLIA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 9.38pg/mL |
Detection Limit: | 15.63~1000pg/mL |
Short Description: | This human TIMP3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Chemiluminescence |
Information
Gene Symbol: | TIMP3 |
Gene ID: | 7078 |
Uniprot ID: | TIMP3_HUMAN |
Specificity: | This kit recognizes Human TIMP-3 in samples. No significant cross-reactivity or interference between Human TIMP-3 and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Description
Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. |
Protein Name | Metalloproteinase Inhibitor 3Protein Mig-5Tissue Inhibitor Of Metalloproteinases 3Timp-3 |
Database Links | Reactome: R-HSA-114608 |
Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
Alternative CLIA Names | Metalloproteinase Inhibitor 3 CLIA kitProtein Mig-5 CLIA kitTissue Inhibitor Of Metalloproteinases 3 CLIA kitTimp-3 CLIA kitTIMP3 CLIA kit |
Specificity | This kit recognizes Human TIMP-3 in samples. No significant cross-reactivity or interference between Human TIMP-3 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
---|---|---|
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 85-100 | 91 |
EDTA plasma(n=8) | 87-99 | 92 |
Cell culture media(n=8) | 97-112 | 103 |
Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
---|---|---|---|---|---|---|
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 53.26 | 118.42 | 407.87 | 48.20 | 110.76 | 424.16 |
Standard deviation | 4.37 | 10.50 | 39.03 | 5.25 | 9.00 | 47.17 |
CV (%) | 8.21 | 8.87 | 9.57 | 10.89 | 8.13 | 11.12 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance