Human Roundabout Homolog 4, ROBO4 ELISA Kit
Cat# E6106Hu-5X96wells
Size : 5X96wells
Brand : Bioassay Technology Laboratory
Product overview
Full product name
Roundabout Homolog 4
Code
E6106Hu
Assay type
Sandwich
Size
48T,96T
Sensitivity
9.26ng/L
Detection range
20-7000ng/L
Sample type
Serum, plasma, cell culture supernates
Species
Human
Storage
2-8ºC
Assay time
1h 30m
Background
Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins (By similarity). Involved in the maintenance of endothelial barrier organization and function (PubMed: 30455415).
UniProt accession
MASS(Da)
107,457
GeneID
54538
Synonyms
Magic roundabout;ROBO 4;ROBO4;Roundabout homolog 4
Gene names
ROBO4
Research area
Cancer
Target protein
ROBO4
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate (25x) | 20ml x1 |
Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6106Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
4000ng/L | 2.075 | 2.223 | 2.194 |
2.37 | |||
2000ng/L | 1.208 | 1.228 | 1.199 |
1.248 | |||
1000ng/L | 0.815 | 0.831 | 0.802 |
0.848 | |||
500ng/L | 0.487 | 0.445 | 0.417 |
0.404 | |||
250ng/L | 0.267 | 0.218 | 0.189 |
0.169 | |||
0ng/L | 0.031 | 0.029 | 0 |
0.026 |
Precision
We measured random samples of E6106Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-Assay | 1 | 18 | 1998.4 | 72.28 | 3.6 |
Intra-Assay | 2 | 18 | 890.1 | 56.93 | 6.4 |
Intra-Assay | 3 | 18 | 1564.6 | 105.13 | 6.7 |
Protocols
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