Human Roundabout Homolog 1, ROBO1 ELISA Kit
Cat# E6105Hu-48wells
Size : 48wells
Brand : Bioassay Technology Laboratory
Product overview
Full product name
Roundabout Homolog 1
Code
E6105Hu
Assay type
Sandwich
Size
48T,96T
Sensitivity
0.025ng/mL
Detection range
0.05-20ng/mL
Sample type
Serum, plasma, cell culture supernates
Species
Human
Storage
2-8ºC
Assay time
1h 30m
Background
Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed: 10102268, PubMed: 24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed: 24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed: 26529257). May be required for lung development (By similarity).
UniProt accession
MASS(Da)
180,930
GeneID
6091
Synonyms
Deleted in U twenty twenty;H-Robo-1;ROBO 1;ROBO1;Roundabout homolog 1
Gene names
ROBO1
Research area
Cancer
Target protein
ROBO1
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate (25x) | 20ml x1 |
Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6105Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
12ng/mL | 2.174 | 2.197 | 2.141 |
2.219 | |||
6ng/mL | 1.538 | 1.48 | 1.424 |
1.421 | |||
3ng/mL | 0.916 | 0.921 | 0.865 |
0.926 | |||
1.5ng/mL | 0.454 | 0.483 | 0.427 |
0.512 | |||
0.75ng/mL | 0.275 | 0.259 | 0.203 |
0.243 | |||
0ng/mL | 0.049 | 0.056 | 0 |
0.062 |
Precision
We measured random samples of E6105Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-Assay | 1 | 18 | 6 | 0.23 | 3.9 |
Intra-Assay | 2 | 18 | 2.4 | 0.12 | 4.9 |
Intra-Assay | 3 | 18 | 3.2 | 0.18 | 5.8 |
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