Human RETN(Resistin) CLIA Kit
Product Details
Properties
Assay type | Sandwich-CLIA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 12.50-800 pg/mL |
Sensitivity | 7.50 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human RETN in samples. No significant cross-reactivity or interference between Human RETN and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 15%. |
Application | This CLIA kit applies to the in vitro quantitative determination of Human RETN concentrations in serum, plasma and other biological fluids. |
Test Principle
Kit components & Storage
Item | Specifications | Storage |
---|---|---|
Micro CLIA Plate(Dismountable) | 96T: 8 wells ×12 strips 48T: 8 wells ×6 strips | -20℃, 12 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 48T: 1 vial, 60 μL | |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 48T: 1 vial, 60 μL | -20℃(Protect from light), 12 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 12 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃(Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Technical Data
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human RETN were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human RETN were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 43.23 | 66.99 | 375.61 | 45.42 | 64.95 | 344.70 |
Standard deviation | 5.26 | 7.31 | 24.64 | 4.88 | 7.20 | 38.78 |
CV (%) | 12.17 | 10.91 | 6.56 | 10.74 | 11.09 | 11.25 |
Recovery
The recovery of Human RETN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 90-105 | 96 |
EDTA plasma(n=8) | 97-113 | 104 |
Cell culture media(n=8) | 86-100 | 92 |
Linearity
Samples were spiked with high concentrations of Human RETN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Target Information
Synonyms | ADSF, FIZZ3, RETN1, RSTN, XCP1 |
Research Area | Cardiovascular,, |
Assay Procedures
1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃. | |
2.Remove the liquid. | |
3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times. | |
4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times. | |
5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃. | |
6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results. |