Human IL-8 ELISA Kit (48-well)
Cat# EA100031
Size : 1x48wells
| Product Data | |
| ACCN | NM_000584 |
|---|---|
| Assay Length | 3 hours |
| Assay Type | Solid Phase Sandwich ELISA |
| Assay Type | Sandwich |
| Background | Interleukin 8 (IL-8), a member of the neutrophil-specific CXC subfamily of chemokines, is a potent neutrophil chemotactic and activating factor. It is a primary inflammatory cytokine produced by many cells including monocytes/ macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, astrocytes and chondrocytes. It is in response to proinflammatory stimuli such as IL-1, TNF, LPS and viruses. Its function is, in part, to attract neutrophils to the site of inflammation and to activate them. The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa). Further proteolysis of the N-terminal end leads to a variant form with 72 amino acids; full activation of IL-8 may require cleavage to the 72 amino acid form. IL-8 can form non-covalent dimers in solution, especially at high concentrations, but dimerization is not necessary for biological activity. IL-8 binds to two seven-transmembrane, G protein-coupled receptors, CXCR1 and CXCR2, as well as to the non-signalling Duffy antigen on red-blood cells. The Duffy antigen may play a role in regulating IL-8 activity on functional receptors. |
| Components |
|
| Curve Range | 15.625-1000pg/ml |
| figure_legend | Representative standard curve for IL-8 ELISA. IL-8 was diluted in serial two-fold steps in Sample Diluent. |
| Format | 48-well strip plate |
| Gene Symbol | CXCL8 |
| Interference | No significant interference observed with available related molecules. |
| Locus ID | 3576 |
| Reactivity | Human |
| Reactivity | Human |
| Reactivity | Human |
| Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
| Sample Volume | 20 uL |
| Sensitivity | 7pg/mL |
| Signal | Colorimetric |
| Species | Human |
| Specificity | Natural and recombinant Human IL-8 Ligand |
| Standard Curve | /assets/images/elisa/kits/128/che0011-048-1-e.jpg |
| Target Symbol | CXCL8 |
| Description | Human IL-8 ELISA Kit (48-well) |