E-EL-H0572-10x96T | Human β-EP(Beta-Endorphin) ELISA Kit Biotrend

Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	50 μL
Total Assay Time	2 h 30 min
Reacitivity	Human
Specificity	This kit recognizes Human β-EP in samples.No significant cross-reactivity or interference between Human β-EP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human β-EP. During the reaction, Human β-EP in the sample or standard competes with a fixed amount of Human β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

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