E-EL-0157-96T | Human Cortisol ELISA Kit Biotrend USA

Product Summary

Sensitivity	2.92 ng/mL
Detection Range	6.25-400 ng/mL
Sample Volume	50 μL
Total Assay Time	2 h 30 min
Reacitivity	Human
Specificity	This kit recognizes Human Cortisol in samples.No significant cross-reactivity or interference between Human Cortisol and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma, urine
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Cortisol. During the reaction, Human Cortisol in the sample or standard competes with a fixed amount of Human Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Cortisol. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Cortisol in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Background

Cortisol is a hormone produced by the adrenal glands in response to physical and emotional stress, and is involved in a variety of homeostasis maintenance mechanisms such as blood pressure regulation, the immune system, protein, carbohydrate and fat metabolism, and anti-inflammatory effects. In addition, cortisol has been implicated in the effects of metabolism and immune system function. In healthy young people, cortisol levels are elevated due to stress, which is associated with dynamic and static balance scores. This suggests that cortisol not only plays an important role in the response to stress, but is also associated with multiple physiological system functions, such as immune function, glucose regulation, vascular tone, and bone metabolism. As a result, cortisol is essential for many functions in the body, including but not limited to regulating the body's response to stress, helping to control the body's use of fat, protein and carbohydrates, suppressing the inflammatory response, and regulating blood pressure and blood sugar, among others. Meanwhile, low cortisol levels have been linked to Addison's disease, while high cortisol levels have been linked to chronic stress and Cushing's syndrome

Research Area

Signal Transduction , Metabolism

Human Cortisol ELISA Kit

Cat# E-EL-0157-96T

Brand : Elabscience

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