Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) HTR3A-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with HTR3A monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with HTR3A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HTR3A monoclonal antibody, clone 4D5E7.
Flow Cytometry
Flow cytometric analysis of Hela cells with HTR3A monoclonal antibody (green) and negative control (red).
- Specifications
Product Description
Mouse monoclonal antibody raised against recombinant human HTR3A.
Immunogen
Recombinant protein corresponding to amino acid 24-157 of human HTR3A from E. coli.
Host
Mouse
Theoretical MW (kDa)
55.3
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:100-1:500)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) HTR3A-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissue with HTR3A monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with HTR3A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of HTR3A monoclonal antibody, clone 4D5E7.Flow Cytometry
Flow cytometric analysis of Hela cells with HTR3A monoclonal antibody (green) and negative control (red). - Gene Info — HTR3A
Entrez GeneID
3359Gene Name
HTR3A
Gene Alias
5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3
Gene Description
5-hydroxytryptamine (serotonin) receptor 3A
Omim ID
182139Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
5-hydroxytryptamine (serotonin) receptor-3|5HT3 serotonin receptor|serotonin receptor|serotonin-gated ion channel receptor
- Interactomes
- Diseases