Images
Immunocytochemistry
Application

Immunocytochemistry

Immunocytochemical staining of HeLa cells with HSP90AA1/HSP90AB1 monoclonal antibody, clone Hyb-K41220A (ATTO 390) (Cat # MAB18058). (A) DAPI (blue) nuclear stain. (B) Anti-HSP90AA1/HSP90AB1 Antibody. (C) Composite.

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against partial recombinant human HSP90AA1/HSP90AB1.

    Immunogen

    Recombinant protein corresponding to amino acids 291-304 of human HSP90 alpha.

    Host

    Mouse

    Reactivity

    Human

    Form

    Liquid

    Conjugation

    ATTO 390

    Purification

    Protein G purification

    Isotype

    IgG2a

    Recommend Usage

    ELISA
    Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.2 (50% glycerol, 0.09% sodium azide).

    Storage Instruction

    Store at -20°C.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunocytochemistry

    Immunocytochemical staining of HeLa cells with HSP90AA1/HSP90AB1 monoclonal antibody, clone Hyb-K41220A (ATTO 390) (Cat # MAB18058). (A) DAPI (blue) nuclear stain. (B) Anti-HSP90AA1/HSP90AB1 Antibody. (C) Composite.

    Immunofluorescence

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — HSP90AA1

    Entrez GeneID

    3320

    Protein Accession#

    P07900;P08238

    Gene Name

    HSP90AA1

    Gene Alias

    FLJ31884, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2

    Gene Description

    heat shock protein 90kDa alpha (cytosolic), class A member 1

    Omim ID

    140571

    Gene Ontology

    Hyperlink

    Gene Summary

    HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM

    Other Designations

    heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|heat shock 90kD protein, alpha-like 4|heat shock 90kDa protein 1, alpha

  • Gene Info — HSP90AB1

    Entrez GeneID

    3326

    Protein Accession#

    P07900;P08238

    Gene Name

    HSP90AB1

    Gene Alias

    D6S182, FLJ26984, HSP90-BETA, HSP90B, HSPC2, HSPCB

    Gene Description

    heat shock protein 90kDa alpha (cytosolic), class B member 1

    Omim ID

    140572

    Gene Ontology

    Hyperlink

    Gene Summary

    HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM

    Other Designations

    OTTHUMP00000016517|OTTHUMP00000016518|OTTHUMP00000016519|OTTHUMP00000039869|heat shock 90kD protein 1, beta|heat shock 90kDa protein 1, beta|heat shock protein beta

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  • Diseases