- More Files
Western Blot (Tissue lysate)
HSD11B1 polyclonal antibody (Cat # PAB6723) (0.3 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of HSD11B1.
Immunogen
A synthetic peptide corresponding to human HSD11B1.
Sequence
CTSYNMDRFINK
Host
Goat
Theoretical MW (kDa)
32.4
Reactivity
Human
Specificity
This antibody is expected to recognize human HSD11B1 protein. Both NP_005516.1 and NP_861420.1 variants encode the same protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (0.3-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Tissue lysate)
HSD11B1 polyclonal antibody (Cat # PAB6723) (0.3 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
- Gene Info — HSD11B1
Entrez GeneID
3290Protein Accession#
NP_005516.1;NP_861420.1Gene Name
HSD11B1
Gene Alias
11-DH, 11-beta-HSD1, HDL, HSD11, HSD11B, HSD11L, MGC13539, SDR26C1
Gene Description
hydroxysteroid (11-beta) dehydrogenase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
11-beta-hydroxysteroid dehydrogenase 1|OTTHUMP00000034649|OTTHUMP00000034650|corticosteroid 11-beta-dehydrogenase isozyme 1|short chain dehydrogenase/reductase family 26C, member 1
- Interactomes
- Pathways
- Diseases
- Publication Reference
- Metabolic syndrome without obesity: Hepatic overexpression of 11beta-hydroxysteroid dehydrogenase type 1 in transgenic mice.
Paterson JM, Morton NM, Fievet C, Kenyon CJ, Holmes MC, Staels B, Seckl JR, Mullins JJ.
PNAS 2004 May; 101(18):7088.
- Metabolic syndrome without obesity: Hepatic overexpression of 11beta-hydroxysteroid dehydrogenase type 1 in transgenic mice.