HotBegan™ Hot Start Green-Taq MasterMix (2x)
Cat# P0721
Size : 10x1.25mL (500rxn)
Brand : Canvax Biotech
HotBegan™ Hot Start Green-Taq MasterMix
For a Specific, Highly Efficient & Reliable Amplifications of DNA up 160 fg, with visual Tracking of DNA Migration
HotBegan™ Hot Start Green-Taq Master Mix (2x) is an optimized ready-to-use solution containing HotBegan™ Hot Start Taq DNA Polymerase, dNTPs, MgCl2 and Stabilizers. It is inactive at room temperature and only requires addition of template, primers, and water.
HotBegan™ Hot Start Taq DNA Polymerase is a Taq DNA Polymerase bound to a proprietary antibody that blocks Polymerase activity until denaturation step occurs. The heat labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and nonspecific products.
MasterMix also contains an Agarose Loading Buffer including a including two tracking dyes (blue and yellow dye) for visual tracking of DNA migration and a dense compound to facilitate the drop-down of the samples into the well agarose gels. The blue dye (migrates with 3 to 5 kb DNA fragments in 1% agarose gel) and the yellow dye (migrates faster than 10 bp DNA fragments in 1% agarose gel).
Like the Taq polymerase, the enzyme has 5’→3’ Polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of enzyme activities are detectable.
Detailed information:
- Time-Saving Protocol: save time in the PCR process and in Agarose loading samples.
- Optimized for TA Cloning and GC Cloning.
- Accurate & Fast visual Tracking of DNA Migration: thanks to its composition that includes Green Dye Buffer.
- High specificity: minimizes unspecific amplification.
- Efficient: prevents or minimizes primer-dimer and nonspecific products.
- Great sensitivity: amplifies from femptograms of DNA targets.
- Inactive: at Room Temperature.
- Powerful: amplification of targets up to 5 kb.
- Assay Conditions: 25mM Tris-HCl pH9.0 at 25 °C, 50mM KCl, 2mM MgCl2, 0.1mg/mL gelatine, 200 μM of dATP, dGTP, dTTP, 100μM[α32-P]dCTP (0.05 μCi/nmol) and 12.5 μg activated salmon sperm DNA.
- Unit Definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.
Includes for 250 rxn:
– 5 x 1.25 mL HotBegan™ Hot Start Green-Taq MasterMix (2x)*
– 1.5 mL 50mM MgCl2 Solution**
* Includes HotBegan™ Hot Start Taq DNA Polymerase, Green Dye buffer (2x), 0.4 mM of each dNTP, 4 mM Mg2+ and 24% Glycerol.
**Separate tube 50 mM MgCl2 solution is provided for further optimisation. In some cases, we recommend to optimize Mg2 + concentration.
Datasheet MSDS- PCR fragments amplification for TA or GC Cloning.
- Design for High Throughput Applications.
- Amplification from a limited DNA template or low copy number genes.
- Functionally tested in PCR.
- Exempt of nucleases (endo-, exo and ribonucleases) activities guaranteed by appropriate quality tests.
- Shipped in: Gel pack.
- Storage: -20 °C.
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.