Reagents and instruments for immunology, cell biology and molecular biology.

Horse Interleukin 1Beta, IL-1B ELISA Kit

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Cat# E0079HO-48wells

Size : 48wells

Brand : Bioassay Technology Laboratory

Horse Interleukin 1Beta, IL-1B ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.091ng/L

Detection range: 0.2-70ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Horse

Product Summary

Size: 96T,48T

Sensitivity: 0.091ng/L

Detection range: 0.2-70ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Horse

Product overview

Full product name

Interleukin 1Beta

Code

E0079HO

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.091ng/L

Detection range

0.2-70ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Horse

Storage

2-8ºC

Assay time

1h 30m

Background

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.

UniProt accession

Q28386

MASS（Da）

30,268

GeneID

1.00034237100052E+17

Synonyms

IL 1B;IL 1beta;IL-1 beta;IL1B;Interleukin-1 beta

Gene names

IL1B

Research area

Immunology; Microbiology; Cardiovascular; Metabolism

Target protein

IL1B

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E0079HO are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/L	2.107	2.183	2.129
	2.26
20ng/L	1.206	1.273	1.218
	1.34
10ng/L	0.906	0.819	0.765
	0.732
5ng/L	0.505	0.458	0.403
	0.411
2.5ng/L	0.277	0.266	0.212
	0.256
0ng/L	0.049	0.055	0
	0.061

Precision

We measured random samples of E0079HO within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	15.2	0.99	6.5
Intra-Assay	2	18	26.8	0.91	3.4
Intra-Assay	3	18	9.3	0.62	6.6

Protocols

> Protocol Booklet

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