HLA Class I ABC mouse monoclonal antibody, clone W6/32, PE
Specifications
Product Data
Clone Name
W6/32
Application
FC
Recommended Dilution
Flow Cytometry: Use 10 μl of neat antibody to label 1x106 cells or 100 μl whole blood. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Reactivity
Bovine, Feline, Human, Monkey, Primate
Antibody Host
Mouse
Isotype
IgG2a
Clonality
Monoclonal
Immunogen
Purified human tonsil lymphocyte membranes. Spleen cells from immunised BALB/c mice were fused with cells of the NS1/1 - Ag4.1 mouse myeloma cell line.
Specificity
Antibody SM1222R recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha-3 and alpha-2 domains (See Ishitani, A. et al. for details). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells.
Buffer
PBS Label: PE State: Lyophilized purified IgG fraction from Tissue Culture Supernatant Stabilizer: 1% BSA, 5% Sucrose Preservative: 0.09% Sodium Azide Label: R. Phycoerythrin (RPE)
Reconstitution Method
Restore with 1ml distilled water
Purification
Affinity Chromatography on Protein G
Conjugation
PE
Storage
Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Stability
Shelf life: one year from despatch.
Shipping
Blue Ice
Background
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
