Histone H3 K9me1 antibody
Cat# orb420396-50ug
Size : 50ug
Brand : Biorbyt
Histone H3 K9me1 antibody
Catalog Number: orb420396
Catalog Number | orb420396 |
---|---|
Category | Antibodies |
Description | Histone H3 K9me1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ChIP, DOT, IF, IHC, Multiplex Assay, WB |
Reactivity | C. elegans, Human, Mouse |
Isotype | IgG |
Immunogen | Histone H3 [Monomethyl Lys9] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 9 of human Histone H3.2. |
Concentration | 0.7 mg/mL |
Dilution range | ChIP: 2-5µg/million cells, IHC: 1:500, IF: 1:500, WB: 1:500 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Histone H3 [Monomethyl Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q71DI3 |
NCBI | NP_001005464 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Alternative names | rabbit anti-Histone H3 monomethyl Lys9 antibody, H |
Note | For research use only |
Application notes | Anti-Histone H3 [Monomethyl Lys9] antibody is tested for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunocytochemistry/Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals. |
Expiration Date | 12 months from date of receipt. |
Chromatin Immunoprecipitation of Histone H3 [Monomethyl Lys9] Antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of Anti-Histone H3 K9Me1 and 20 ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
Dot Blot of Rabbit Histone H3 [Monomethyl Lys9] Antibody. Lane 1: K9 KMe1. Lane 2: K9 KMe2. Lane 3: K9 KMe3. Lane 4: K9. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Monomethyl Lys9] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
Immunofluorescence of Rabbit Anti-Histone H3 [Monomethyl Lys9] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Monomethyl Lys9] antibody at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10000 for 45 min at RT. Localization: Histone H3 [Monomethyl Lys9] is nuclear and chromosomal. Staining: Histone H3 [Monomethyl Lys9] is expressed in green and the nuclei and actin are counterstained with DAPI (blue) and Phalloidin (red).
Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys9] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys9] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys9] Antibody. Lane 1: NIH-3T3 histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys9] at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.