Histone H3 K37me1 antibody
Cat# orb420417-50ug
Size : 50ug
Brand : Biorbyt
Histone H3 K37me1 antibody
Catalog Number: orb420417
| Catalog Number | orb420417 |
|---|---|
| Category | Antibodies |
| Description | Histone H3 K37me1 antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ChIP, DOT, IF, IHC, Multiplex Assay, WB |
| Reactivity | C. elegans, Human, Mouse |
| Isotype | IgG |
| Immunogen | Histone H3 [Monomethyl Lys37] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic methylated peptide surrounding Lysine 37 of human Histone H3. |
| Concentration | 1.0 mg/mL |
| Dilution range | WB: 1µg/mL |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Anti-Histone H3 [Monomethyl Lys37] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined. |
| Conjugation | Unconjugated |
| UniProt ID | Q71DI3 |
| NCBI | NP_001005464 |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | rabbit anti-Histone H3 monomethyl Lys37 antibody, |
| Note | For research use only |
| Application notes | Anti-Histone H3 [Monomethyl Lys37] antibody is tested for Bot Blot, Western Blot, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Anti-Histone H3 is also useful for Dot Blot. Epi-Plus antibody production in collaboration with Novus Biologicals. |
| Expiration Date | 12 months from date of receipt. |
Dot Blot of Rabbit Histone H3 [Monomethyl Lys37] Antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Monomethyl Lys37] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
Dot Blot of Rabbit Histone H3 [Monomethyl Lys37] Antibody. Lane 1: K37me1. Lane 2: K36me1. Lane 3: K36me2. Lane 4: K36me3. Lane 5: K36ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Monomethyl Lys37] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
Immunofluorescence of Anti-Histone H3 K37 me1: Histone H3 monomethyl Lys37 antibody was tested at 1:200 in HeLa cells with FITC (green). Cells and nuclei were counterstained with DAPI (blue) and DyLight 550 (red). (40X).
Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys37] Antibody. Lane 1: HeLa histone prep. Lane 2: NIH-3T3 histone prep. Lane 3: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Methyl Lys37] at 1 µg/ml for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.