Histone Deacetylase (HDAC) BioAssay™ Sample Kit

The histone tail acetylation promotes transcriptional activation. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription. HAT complexes interact with sequence-specific activator proteins and target specific genes. In addition to histones, some HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes. In another respect, histone deacetylation is involved in silencing of gene transcription. Mammalian histone deacetylases can be divided into two classes on the basis of their similarity to various yeast histone deacetylases. The first class is represented by its closeness to the yeast Rpd3-like proteins (HDAC1, -2, -3 and -8), and the second class, which has similarities to yeast Hda1-like proteins (HDAC4, -5 and -7). The Histone Deacetylase (HDAC) Antibody Sampler Kit provides a fast and economical means to evaluate the endogenous levels of HDACs. The kit contains enough primary and secondary antibodies to perform four mini-blot experiments.||Kit Components:|The HDAC antibodies are supplied at 20ul each. The IgG-HR{P components are supplied at 100ul each.||Antibody Reactivity Isotype MW Applications|HDAC1 Mouse mAb H M R Mk IgG1 62kD WB IP IF-IC|HDAC2 Mouse mAb H M R Mk IgG1 62kD WB IP IF-IC|HDAC3 Mouse mAb H M R Mk IgG1 49kD WB IP IF-IC|HDAC4 Mouse mAb H M R Mk IgG2a 140kD WB IP |HDAC5 Rabbit pAb H M R Mk IgG 124kD WB IP IHC-P|HDAC7 Rabbit pAb H M R Mk IgG 120kD WB IP IHC-P|IgG-HRP Gt x Rb pAb IgG|IgG-HRP Horse x Rb pAb IgG||Species Abbreviations:|H= human; M = mouse; R = rat; Mk = monkey; Mk = monkey; Gt = goat|Applications Abbreviations:|WB = Western Blot; IP = Immunoprecipitation; IF-IC = Immunfluorescence-Immunocytochemistry; IHC-P = Immunohistochemistry (paraffin)