Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western Blot (Cell lysate) analysis of HeLa cell lysate.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining of mouse colon.

  • Specifications

    Product Description

    Rabbit monoclonal antibody raised against synthetic peptide of human HIST1H3A (mono methyl R17).

    Immunogen

    A synthetic peptide corresponding to human HIST1H3A (mono methyl R17).

    Host

    Rabbit

    Reactivity

    Human, Mouse, Rat

    Form

    Liquid

    Purification

    Affinity purification

    Isotype

    IgG

    Recommend Usage

    Immunocytochemistry (1:100-250)
    Immunofluorescence (1:100-250)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-250)
    Western Blot (1:100-2000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).

    Storage Instruction

    Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western Blot (Cell lysate) analysis of HeLa cell lysate.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical staining of mouse colon.

    Immunocytochemistry

    Immunofluorescence

  • Gene Info — HIST1H3A

    Entrez GeneID

    8350

    Protein Accession#

    P68431

    Gene Name

    HIST1H3A

    Gene Alias

    H3/A, H3FA

    Gene Description

    histone cluster 1, H3a

    Omim ID

    602810

    Gene Ontology

    Hyperlink

    Gene Summary

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq

    Other Designations

    H3 histone family, member A|histone 1, H3a

  • Interactomes
  • Pathways