Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60) (BSA & Azide Free) (HRP)
Cat# H1830-75X-HRP-100ul
Size : 100ul
Brand : US Biological
H1830-75X-HRP Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60) (BSA & Azide Free) (HRP) discontinued
Clone Type
PolyclonalHost
mouseSource
humanIsotype
IgG1Grade
Affinity PurifiedApplications
E IF IHC IP WBCrossreactivity
Ch Hm Hu Ma Mo RtShipping Temp
Blue IceStorage Temp
-20°CA wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of heat shock proteins (hsp’s) or stress response proteins (srp’s). hsp60 is a potential antigen in a number of autoimmune diseases including human arthritis. H1830-75X is useful in distinguishing hsp60 from mammals and bacteria. ||Applications:|Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.||Recommended Dilutions:|Immunoprecipitation (Native): 2ug/mg protein lysate using Protein G.|Western Blot: 1-2ug/ml for 2 hrs at RT|Immunohistochemistry (Formalin/paraffin): 1:50 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.|Optimal dilutions to be determined by the researcher.||Positive Control:|BT474 cells, MAD109 cells and breast carcinoma||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.