Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60) (BSA & Azide Free) (HRP)

Cat# H1830-75X-HRP-100ul

Size : 100ul

Brand : US Biological

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H1830-75X-HRP Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60) (BSA & Azide Free) (HRP) discontinued

Clone Type
Polyclonal
Host
mouse
Source
human
Isotype
IgG1
Grade
Affinity Purified
Applications
E IF IHC IP WB
Crossreactivity
Ch Hm Hu Ma Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of heat shock proteins (hsp’s) or stress response proteins (srp’s). hsp60 is a potential antigen in a number of autoimmune diseases including human arthritis. H1830-75X is useful in distinguishing hsp60 from mammals and bacteria. ||Applications:|Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.||Recommended Dilutions:|Immunoprecipitation (Native): 2ug/mg protein lysate using Protein G.|Western Blot: 1-2ug/ml for 2 hrs at RT|Immunohistochemistry (Formalin/paraffin): 1:50 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.|Optimal dilutions to be determined by the researcher.||Positive Control:|BT474 cells, MAD109 cells and breast carcinoma||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 0.N.322|Host: mouse|Source: human|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein G affinity chromatography.|Immunogen: Recombinant HSP60 protein. Cellular Localization: Cytoplasmic. Epitope: aa383-447 |Specificity: Recognizes human Heat Shock Protein 60 at ~60kD. Species Crossreactivity: mouse, rat, hamster, mammal and chicken. Does not react with bacteria, helminths or spinach.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant HSP60 protein. Cellular Localization: Cytoplasmic. Epitope: aa383-447
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes human Heat Shock Protein 60 at ~60kD. Species Crossreactivity: mouse, rat, hamster, mammal and chicken. Does not react with bacteria, helminths or spinach.
References
1. Hirata, D., Hirai, I., Iwamoto, M., Yoshio, T., Takeda, A., Masuyama, J.I., Mimori, A., Kano, S., Minota, S., Preferential binding with Escherichia coli hsp60 of antibodies prevalent in sera from patients with rheumatoid arthritis. Clinical Immunology and Immunopathology 82(2): 141-8 (1997, Feb). 2. Mobius, J., Groos, S., Meinhardt, A., Seitz, J., Differential distribution of the mitochondrial heat-shock protein 60 in rat gastrointestinal tract. Cell and Tissue Research 287(2): 343-50 (1997, Feb). 3. Soltys, B.J., Gupta, R.S., Cell surface localization of the 60kD heat shock chaperonin protein (hsp60) in mammalian cells. Cell Biology International 21(5): 315-20 (1997, May). 4. Werner, A., Meinhardt, A., Seitz, J., Bergmann, M., Distribution of heat-shock protein 60 immunoreactivity in testes of infertile men. Cell and Tissue Research 288(3) :539-44 (1997, Jun). 5. Alevy, Y.G., Brennan, D., Durriya, S., Howard, T., Mohanakumar, T., Increased expression of the HDJ-2 heat shock protein in biopsies of human rejected kidney. Transplantation 61(6): 963-7 (1996). 6. Barker, R.N., Wells, A.D., Ghoraishian, M., Easterfield, A.J., Hitsumoto, Y., Elson, C.J., Thompson, S.J., Expression of mammalian 60kD heat shock protein in the joints of mice with pristane-induced arthritis. Clinical and Experimental Immunology 103(1): 83-8 (1996, Jan). 7. Beimnet, K., Soderstrom, K., Jindal, S., Gronberg, A., Frommel, D., Kiessling, R., Induction of heat shock protein 60 expression in human monocytic cell lines infected with Mycobacterium leprae. Infection and Immunity 64(10): 4356-8 (1996, Oct). 8. Hettinga, J.V., Lemstra, W., Meijer, C., Los, G., de Vries, E.G., Konings, A.W., Kampinga, H.H., Heat-shock protein expression in cisplatin-sensitive and-resistant human tumor cells. International Journal of Cancer 67(6): 800-7 (1996, Sep 17). 9. Kligman, I., Grifo, J.A., Witkin, S.S., Expression of the 60kD heat shock protein in peritoneal fluids from women with endometriosis: implications for endometriosis-associated infertility. Human Reproduction 11(12): 2736-8 (1996). 10. Mallard, K., Jones, D.B., Richmond, J., McGill, M., Foulis, A.K., Expression of the human heat shock protein 60 in thyroid, pancreatic, hepatic and adrenal autoimmunity. Journal of Autoimmunity 9(1): 89-96 (1996).