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> Goat anti-NQO1

Goat anti-NQO1

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Cat# EB05370

Size : 100ug

Brand : Everest Biotech

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Code Name Applications Availability Size Tested species Grade
EB05370 Goat Anti-NQO1 Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human, Rat
EB05370 1
EB05370 (0.3µg/ml) staining of U251 (A) and HepG2 (B) cell lysate. (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05370 2
EB05370 (0.3µg/ml) staining of Human Kidney (A), Lung (B) and Duodenum (C) lysate. (35µg protein in RIPA buffer). Detected by chemiluminescence
EB05370 3
EB05370 (0.3µg/ml) staining of Rat Kidney (A), Lung (B) and Duodenum (C) lysate. (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05370 4
EB05370 (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05370 5
EB05370 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Target Protein

Principal Names: NQO1, NAD(P)H dehydrogenase, quinone 1, DTD, QR1, DHQU, DIA4, NMOR1, NMORI, diaphorase-4, diaphorase (NADH/NADPH), diaphorase (NADH/NADPH) (cytochrome b-5 reductase), NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1, DT-diaphorase, NAD(P)H menadione oxidoreductase 1, dioxin-inducible, NAD(P)H:Quinone acceptor oxidoreductase type 1, NAD(P)H menadione oxidoreductase 1, dioxin-inducible, NAD(P)H:quinone oxireductase, azoreductase, diaphorase (NADH/NADPH) (cytochrome b-5 reductase), dioxin-inducible 1, menadione reductase, phylloquinone reductase, quinone reductase 1
Official Symbol: NQO1
Accession Number(s): NP_000894.1; NP_001020604.1; NP_001020605.1
Human GeneID(s): 1728
Non-Human GeneID(s): 18104 (mouse)
Important Comments: This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).

Immunogen

Peptide with sequence C-SIPTDNQIKARK, from the C Terminus of the protein sequence according to NP_000894.1; NP_001020604.1; NP_001020605.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 30kDa band observed in lysates of cell lines U251 and HepG2 and in Human Kidney, Lung and Duodenum lysates, and approx. 30-32kDa band in Rat Kidney, Lung and Duodenum lysates (calculated MW of 30.9kDa according to Human NP_000894.1 and Rat NP_058696.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Kidney shows specific staining of the glomeruli. Recommended concentration: 2-4µg/ml.

Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of HepG2 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Rat, Dog, Pig