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> Goat anti-Insulysin / Insulinase, Biotinylated

Goat anti-Insulysin / Insulinase, Biotinylated

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Cat# EB07898-B

Size : 100ug

Brand : Everest Biotech

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Code Name Applications Availability Size Tested species Grade
EB07898-B Goat Anti-Insulysin / Insulinase, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human
EB07898-B 1
Biotinylated EB07898 (0.3µg/ml) staining of K562 lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Target Protein

Principal Names: IDE, insulin degrading enzyme, INSULYSIN, Abeta-degrading protease, insulin protease, insulinase
Official Symbol: IDE
Accession Number(s): NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1
Human GeneID(s): 3416
Important Comments: This antibody is expected to recognize all reported isoforms (NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1). Reported variants represent identical protein: NP_001309725.1, NP_001309724.1

Immunogen

Peptide with sequence CQQYNFDRDNTE., from the internal region of the protein sequence according to NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog