Reagents and instruments for immunology, cell biology and molecular biology.
 
> Goat anti-Hamartin / TSC1 (isoform 1)

Goat anti-Hamartin / TSC1 (isoform 1)

Print Print
Please log in

Cat# EB07221

Size : 100ug

Brand : Everest Biotech

Request more information

Code Name Applications Availability Size Tested species Grade
EB07221 Goat Anti-Hamartin / TSC1 (isoform 1) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human
EB07221 1
EB07221 (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07221 2
EB07221 (3.75µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07221 3
EB07221 (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Target Protein

Principal Names: TSC1, hamartin, tuberous sclerosis 1, HGNC:12362, KIAA0243, LAM, MGC86987, TSC, tuberous sclerosis 1 protein, tumor suppressor
Official Symbol: TSC1
Accession Number(s): NP_000359.1
Human GeneID(s): 7248
Non-Human GeneID(s): 64930 (mouse)
Important Comments: This antibody is expected to recognise isoform 1 (NP_000359.1) only.

Immunogen

Peptide with sequence C-QLHIMDYNETHHEH, from the C Terminus of the protein sequence according to NP_000359.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22;9(12):1721-7; PMID: 10915759). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Uterus and Liver. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog