Reagents and instruments for immunology, cell biology and molecular biology.
 
> Goat anti-Aminopeptidase A

Goat anti-Aminopeptidase A

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Cat# EB06798

Size : 100ug

Brand : Everest Biotech

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Code Name Applications Availability Size Tested species Grade
EB06798 Goat Anti-Aminopeptidase A Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat
EB06798 1
EB06798 staining (1µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescence.
EB06798 2
EB06798 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Target Protein

Principal Names: aminopeptidase A, ENPEP, glutamyl aminopeptidase (aminopeptidase A), HGNC:3355, APA, gp160, CD249, glutamyl aminopeptidase
Official Symbol: ENPEP
Accession Number(s): NP_001968.3
Human GeneID(s): 2028
Non-Human GeneID(s): 13809 (mouse)

Immunogen

Peptide with sequence C-EQYQKTSLAQEKEK, from the internal region of the protein sequence according to NP_001968.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 170kDa band observed in Human Brain and Testis lysates (calculated MW of 109kDa according to NP_001968.2). Primary incubation was for 1 hour.The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ando et al, J Clin Endocrinol Metab. 2002 May;87(5):2303-9; PMID: 11994381). Recommended concentration: 1-3µg/ml. This antibody has been successfully used in WB on Rat: PMID: 28399178 and 25470983.
IHC: In paraffin embedded Human Kidney shows staining of basement membrane and the mesangium in the glomerulus. Recommended concentration: 3-5µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific References

This antibody has been successfully used in WB on Rat:
Quesada A, Segarra AB, Montoro-Molina S, de Gracia MD, Osuna A, O'Valle F, Gómez-Guzmán M, Vargas F, Wangensteen R.
Glutamyl aminopeptidase in microvesicular and exosomal fractions of urine is related with renal dysfunction in cisplatin-treated rats.
PLoS One. 2017 Apr 11;12(4):e0175462
PMID: 28399178

This antibody has been successfully used in WB on Rat:
Montoro-Molina S, Quesada A, Zafra-Ruiz PV, O'Valle F, Vargas F, de Gracia Mdel C, Osuna A, Wangensteen R
Immunological detection of glutamyl aminopeptidase in urine samples from cisplatin-treated rats.
Proteomics Clin Appl. 2015 Jun;9(5-6):630-5. 
PMID: 25470983