Goat anti-ALDH5A1 (aa 485-496)
Cat# EB10435
Size : 100ug
Brand : Everest Biotech
| Code | Name | Applications | Availability | Size | Tested species | Grade |
|---|---|---|---|---|---|---|
| EB10435 | Goat Anti-ALDH5A1 (aa 485-496) Antibody | Pep-ELISA, WB, IHC | 100µg specific antibody in 200µl | Human, Mouse, Rat |
EB10435 (0.5µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10435 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Target Protein
Principal Names: aldehyde dehydrogenase 5 family, member A1, aldehyde dehydrogenase 5A1, mitochondrial succinate semialdehyde dehydrogenase, NAD(+)-dependent succinic semialdehyde dehydrogenase, SSADH, SSDH, succinate-semialdehyde dehydrogenase, ALDH5A1Official Symbol: ALDH5A1
Accession Number(s): NP_733936.1; NP_001071.1
Human GeneID(s): 7915
Non-Human GeneID(s): 214579 (mouse), 291133 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_733936.1; NP_001071.1). Amino acid numbering in name calculated from NP_733936.1.
Immunogen
Peptide with sequence C-SQDPAQIWRVAE, from the internal region of the protein sequence according to NP_733936.1; NP_001071.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 55kDa band observed in Human Liver lysates (calculated MW of 58.7kDa according to NP_733936.1). Recommended concentration: 0.3-1µg/ml. Please note that both this product and EB10436 detects a 50kDa in Mouse and Rat Liver lysates (calculated MW of 56.0kDa according to Mouse NP_766120.1).
IHC: In paraffin embedded Human Liver shows heavy textured cystoplasm staining in hepatocytes, consistent with mitochondia.. Recommended concentration, 5-10µg/ml.
Species Reactivity
Tested: Human, Mouse, RatExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol
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