Reagents and instruments for immunology, cell biology and molecular biology.
 
> Glycogen Fluorometric Assay Kit

Glycogen Fluorometric Assay Kit

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Cat# E-BC-F040-96T

Size : 96T

Brand : Elabscience

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Detection Principle Glycogen produces glucose under the action of starch glycosidase, and glucose is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of the peroxidase, hydrogen peroxide be oxidized to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm is proportional to the glycogen content.
Sample Type Animal liver,Muscle
Detection Method Fluorometric method
Detection Instrument Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
Research Area Glycolysis And Lipid Metabolism ,  Energy Metabolism
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.06 μg/mL
Detection Range 0.06-4.0 μg/mL
Precision inter-assay CV: 6.6% | intra-assay CV: 3.4%
Assay Time 50 min
Cat.No. Product Name Sizes
E-BC-K073-S Glycogen Colorimetric Assay Kit (Liver/Muscle Samples) 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Rat liver tissue homogenate 3000-5000
10% Mouse muscle tissue homogenate 10-20

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor® Violet 450

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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detection instrument

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