Reagents and instruments for immunology, cell biology and molecular biology.

GFAP Monoclonal Antibody

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Cat# E-AB-22022-120

Size : 120uL

Brand : Elabscience

Verified Samples	Verified Samples in WB: Rat brain Verified Samples in IHC: Mouse kidney Verified Samples in IF: Mouse brain
Dilution	WB 1:500-1:5000, IHC 1:50-1:300, IF 1:100-1:300
Isotype	IgG
Host	Mouse
Reactivity	Mouse, Rat
Applications	WB, IHC-p, IF
Clonality	Monoclonal
Immunogen	Synthetic Peptide
Abbre	GFAP
Synonyms	ALXDRD, FLJ42474, FLJ45472, GFAP, Glial fibrillary acidic protein, Intermediate filament protein, cb345, etID36982.3, gfapl, wu:fb34h11, wu:fk42c12, xx:af506734, zgc:110485
Swissprot	P14136
Calculated MW	50 kDa
Observed MW	45 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Cytoplasm
Tissue Specificity	Expressed in cells lacking fibronectin
Concentration	1 mg/mL
Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method	Protein A purification
Research Areas	Cancer, Neuroscience, Signal Transduction, Stem Cells, Tags and Cell Markers
Clone No.	3H1
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background	This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

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E-AB-1015	Goat Anti-Mouse IgG(H+L)(FITC conjugated)	500μL , 120μL , 60μL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500μL , 120μL , 60μL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500μL , 120μL , 60μL
E-AB-1059	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated)	500μL , 120μL , 60μL
E-AB-1076	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated)	500μL , 120μL , 60μL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500μL , 120μL , 1mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50mL , 18mL , 6mL , 3mL
E-IR-R220	Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50Assays