Western Blot (Tissue lysate)
Western blot analysis (tissue lysate) of mouse whole brain lysates showing specific immunolabeling of the β-subunit of the GABAA-R.
- Specifications
Product Description
Rabbit polyclonal antibody raised against a recombinant Gabrb1.
Immunogen
Recombinant protein corresponding to rat Gabrb1.
Host
Rabbit
Theoretical MW (kDa)
55
Reactivity
Bovine, Chicken, Dog, Human, Mouse, Primates, Rat, Xenopus, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. - Applications
Western Blot (Tissue lysate)
Western blot analysis (tissue lysate) of mouse whole brain lysates showing specific immunolabeling of the β-subunit of the GABAA-R. - Gene Info — Gabrb1
Entrez GeneID
25450Protein Accession#
P15431Gene Name
Gabrb1
Gene Alias
GARB1
Gene Description
gamma-aminobutyric acid (GABA) A receptor, beta 1
Gene Ontology
HyperlinkGene Summary
O
Other Designations
Gamma-aminobutyric acid receptor beta 1|gamma-aminobutyric acid (GABA-A) receptor, subunit beta 1|gamma-aminobutyric acid receptor, subunit beta 1
- Publication Reference
- Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABA(A) receptor gamma2 subunit.
Ogris W, Poltl A, Hauer B, Ernst M, Oberto A, Wulff P, Hoger H, Wisden W, Sieghart W.
Biochemical Pharmacology 2004 Oct; 68(8):1621.
- Subunit composition and quantitative importance of GABA(A) receptor subtypes in the cerebellum of mouse and rat.
Poltl A, Hauer B, Fuchs K, Tretter V, Sieghart W.
Journal of Neurochemistry 2003 Dec; 87(6):1444.
Application：IP, WB, Human, Mouse, Rat, HEK 293 cells, Mouse and Rat cerebellar extracts.
- Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABA(A) receptor alpha1 subtype.
McKernan RM, Rosahl TW, Reynolds DS, Sur C, Wafford KA, Atack JR, Farrar S, Myers J, Cook G, Ferris P, Garrett L, Bristow L, Marshall G, Macaulay A, Brown N, Howell O, Moore KW, Carling RW, Street LJ, Castro JL, Ragan CI, Dawson GR, Whiting PJ.
Nature Neuroscience 2000 Jun; 3(6):587.
- Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABA(A) receptor gamma2 subunit.