Product Description  

FastPure EndoFree Plasmid Maxi Kit is suitable for extraction from 150 - 300 ml of bacterial solution cultured overnight, using an improved SDS-alkaline lysis method to lyse the bacteria. The crude extract is selectively combined with a unique Endotoxin Scavenger and separated by centrifugation to remove endotoxins. Then, the silica gel membrane selectively binds to plasmid DNA in the solution under conditions of high-salt and low-pH. This is followed by addition of wash buffer to remove impurities and other bacterial components. Finally, a low-salt, high-pH elution buffer is used to elute pure plasmid DNA from the silicon matrix membrane. The silica gel membrane employs special adsorption membrane, and the adsorption amount difference between the column and the column is very small and the repeatability is good. Phenol, chloroform and other toxic reagents are not required, and neither is ethanol precipitation steps. This kit can be used to rapidly extract 0.2 - 1.5 mg of pure high-copy plasmid DNA, with an extraction rate of 80% - 90%. The kit uses an unique process formula removes endotoxin, the content of endotoxin is extremely low and the cell transfection effect is excellent. The extracted plasmid could be directly used in enzyme digestion, PCR, in vitro transcription, transformation, sequencing and other molecular biology experiments.

 Features  

• Transfection level : Extremely low endotoxin content (< 0.1 EU/μg DNA)

• High yield: The yield of high copy plasmid is 0.2 mg-1.5 mg

• Wide compatible: The input amount of bacterial cultures is 50 - 300 ml 

 Components  

 Storage  

RNase A should be stored at -30 ~ -15°C and transported at ≤0°C.

Endotoxin Scavenger can be stored at 2 ~ 8°C for one month, stored at -30 ~ -15°C for long-term storage and transported at ≤0°C.

Other components should be stored at room temperature (15 ~ 25°C) and transported at room temperature.