This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against Human IgA, anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Conjugation
HRP
Storage
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives
None. Do NOT add sodium azide!
Alternative names
Goat F(ab')2 anti-Human IgA (alpha chain) Antibody
Note
For research use only
Application notes
This antibody is supplied in 0.01M Sodium Phosphate, 0.25M Sodium Chloride pH: 7.6. F(ab')2 Anti-Human IgA Peroxidase Antibody is designed for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
