ExoTEST is a sandwich enzyme-linked immunoassay for in-vitro exosome quantification in human urine.
| Sample type: | Urine |
| Starting material: | Up to 100 ul of biological sample can be loaded per well. Unfractionated plasma sample can be directly used for capture. Concentrated urine samples are recommended prior capture according to our suggested protocol. |
| Assay type: | Sandwich enzyme-linked immunoassay. |
| Sensitivity: | The detection limit of an assay is lower than 0.35 ug of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein. |
| Product overview: | ExoTEST Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from biological samples such as plasma and urine. ExoTEST Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely α-CD9. Secondary antibodies and substrate reagents are included in the kit. Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage. ExoTEST ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies. |
| Primary antibody: | α-CD9 mouse antibody |
| Exosome standard: | Lyophilized exosomes from urine of healthy donors (see details). |
| Assay protocol: | Kit contains all the reagents necessary for exosome capture and protein targeted detection. Assay calibration: Kit contains exosome standards for assay calibration. Depended on the sample type used, kit may include human plasma, serum or urine exosomes. All exosome standards are lyophilized and quantified for overall protein content and exosomes number. This standards enable both absolute and relative quantification exosomes in analyzed samples. |
| Shipment and storage: | All the reagents are shipped at 4C and storage conditions as recommended in the product insert. |
| Expiration date: | 12 months. |
Application :
Exosome capture and quantification using the ExoTEST ensures dose-dependent exosome detection in a variety of samples. The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.
Comprehensive exosome profiling by implementing different combinations of capture and detection antibodies. ExoTEST can be customized for assessing multiple antigens in a total exosome population within a sample. Informative protein profiles for the samples of interest can be combined with subsequent nucleic acid (mRNA or miRNA) extraction and downstream analysis from captured exosomes.
Specific diagnostic applications using the ExoTEST allows the quantitative assessment of total circulating exosomes and specific exosomal sub-populations, defined by differentially expressed antigens under normal and pathological conditions.