IC50: Not available.

EDC.HCl, which is commonly regarded as carbodiimide reagents, serves as a very useful tool for the formation of amide bonds (peptide bonds) in an aqueous medium. As one of the carbodiimide reagents, it is also effectively applied in anhydroxydation, polynucleotide synthesis, esterification and lactonization.In addition, EDC.HCl is also widely used as water soluble condensing reagent. [1]

In vitro: Carbodiimide-mediated coupling method was one of the most traditional approaches for peptide-bond formation. With the aim to synthesis target compounds, carbodiimide reagents were added after thoroughly mixing of a carboxylic acid and an amine. After the reaction was accomplished, the carbodiimide reagent was then changed into its corresponding urea. [2] Recently, a simple and fast detection method using spectrophotometric flow injection analysis was developed. This method was based on the reaction between EDC.HCl and pyridine in acidic aqueous solution. After reaction at 40C for a while, the absorbance was measured at 400 nm. The calibration curve demonstrated a good linearity from 0 to 10% of EDC × 104x (x, % concentration of EDC]

In vivo: So far, no in vivo data has been reported.

Clinical trial: So far, no clinical trial has been conducted.

References:
[1]Kunihiko Seno, Kazuki Matumura, Mitsuko Oshima, and Shoji Motomizu.-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow injection analysis. Anal Sci. 2008 Apr; 24: 505-8.
[2] Jad YE, Khattab SN, Beatriz G. May; 2015(14): 3116-20.