EasyPure® Genomic DNA Kit (with RNase A）
Cat# EE101-01
Size : 50rxns
Brand : TransGen Biotech
ManualEasyPure® Genomic DNA Kit provides a simple and convenient way to efficiently isolate high-quality genomic DNA from a variety of materials (mammalian cells, tissues, mouse tails, E. coli and yeast). Cells and tissues are enzymatically lysed. DNA is specifically bound to silica-based column. The isolated DNA is suitable for PCR, restriction enzyme digestion and Southern blot, etc.
• Fast extraction, high yield (up to 15 μg).
• High integrity of cDNA ensured by mild lysis conditions without physical disruption, thus reducing damage to gDNA during cell lysis process.
• High purity enabled by spin column which can efficiently and specifically bind to DNA and removs protein, salts, lipids or other contaminants.
Some samples extracted using EE101
Mouse Samples | Concentration (ng/μl) | A260/A280 | A260/A230 |
Uterus(01) Ovary(02) Kidney(03) liver (04) lungs(05) Heart (06) Brain (07) Spleen (08) Mesenteric lymph nodes (09) Inguinal lymph nodes (10) Drug delivery site (11) Skin(12) Sciatic nerve (13) Bone marrow (14) Spinal cord (15) Blood (16) | 173.92 120.39 130.77 141.34 93.51 111.63 90.23 464.25 637.62 67.87 165.77 238.27 20.73 109.58 79.54 60.40 | 1.92 1.92 1.93 1.93 1.93 1.92 1.95 1.95 1.95 1.94 1.92 1.93 1.93 1.91 1.94 2.13 | 2.3 2.26 2.1 2.12 2.19 2.05 1.94 2.42 2.35 1.75 2.14 2.27 1.82 2.09 2.13 1.22 |
| Cynomolgus monkey samples | Concentration (ng/μl) | A260/A280 | A260/A230 |
Testicles (01) Epididymis (02) Kidney(03) liver (04) lungs(05) Heart (06) Brain (07) Spleen (08) Mesenteric lymph nodes (09) Inguinal lymph nodes (10) Drug delivery site(11) Skin(12) Bone marrow (13) Bone marrow (thoracic )(14) Sciatic nerve (drug delivery site) (15) Salivary glands (16) Lacrimal gland (17) | 1248.48 824.18 357.34 199.15 323.04 144.98 81.67 518.42 670.75 1600.92 282.61 327.76 34.68 72.02 256.43 235.87 230.25 | 1.91 1.89 1.92 1.85 1.91 1.92 1.92 1.93 1.85 1.93 1.92 1.90 1.93 1.67 1.91 1.90 1.93 | 2.27 2.35 1.66 1.07 2.07 2.11 1.51 2.21 1.27 2.36 1.96 1.41 1.32 0.57 1.85 1.61 2.22 |
A total of nearly 8,000 samples were extracted using EE101, including samples such as urine, saliva, and skin swabs in addition to the above-mentioned sample types.
Storage
at room temperature (15-25°C) in a dry place for one year.
Shipping
at room temperature.
| Component | EE101-01 (50 rxns) | EE101-02 (200 rxns) | EE101-11 (50 rxns) | EE101-12 (200 rxns) |
| Lysis Buffer 2 (LB2) | 6 ml | 24 ml | 6 ml | 24 ml |
| Binding Buffer 2 (BB2) | 28 ml | 110 ml | 28 ml | 110 ml |
| Clean Buffer 2 (CB2) | 15 ml | 60 ml | 15 ml | 60 ml |
| Wash Buffer 2 (WB2) | 12 ml | 2×22 ml | 12 ml | 2×22 ml |
| Elution Buffer (EB) | 25 ml | 80 ml | 25 ml | 80 ml |
| RNase A (20 mg/ml) | 1 ml | 4×1 ml | 0 | 0 |
| Proteinase K (20 mg/ml) | 1 ml | 4×1 ml | 1 ml | 4×1 ml |
| Genomic Spin Column with Collection Tubes | 50 each | 200 each | 50 each | 200 each |
Characterization of a novel strain phylogenetically related to Kocuria rhizophila and its chemical modification to improve performance of microbial fuel cells[J]. Biosensors and Bioelectronics, 2015, 69:113-120.
Xu X , Wang S , Liu J , et al. Hypoxia induces downregulation of soluble guanylyl cyclase β1 by miR-34c-5p[J]. Journal of Cell Science, 2012, 125(24):6117-6126.
Geng J , Wang X , Wei H , et al. Efficient Attenuation of NK Cell-Mediated Liver Injury through Genetically Manipulating Multiple Immunogenes by Using a Liver-Directed Vector[J]. The Journal of Immunology, 2013, 190(9):4821-4829.
Yao P , Chen X , Yan Y , et al. Glutaredoxin 1, glutaredoxin 2, thioredoxin 1, and thioredoxin peroxidase 3 play important roles in antioxidant defense in Apis cerana cerana[J]. Free Radical Biology and Medicine, 2014, 68:335-346.
Luo J , Yang J , He H , et al. A new electrochemically active bacterium phylogenetically related to Tolumonas osonensis and power performance in MFCs[J]. Bioresource Technology, 2013, 139:141-148.